Issn tlc and hptlc fingerprint profiles of different. Hptlc finger print analysis of phytocompounds and in vitro. Hindu college, nagercoil 629 002, kanyakumari dist. The medicinal value of the plants lies in the bioactive phytochemical constituents that produce definite physiological effects on human body. Phenol and saponin were present in only methanol extracts of leaves and flowers. Vitex negundo medicinal plant anylisis by morden hptlc technique db dupare. Less volatile constituents are separated by this technique. Preliminary phytochemical screening was performed using various biochemical tests. Several studies, in vitro, preclinical, and clinical have confirmed that this class of compounds displays a wide array of very important pharmacological properties.
Hptlc fingerprint profile of leaf and stem methanolic extract. The antioxidant activities of the leaves in ethanol extract are assessed. Qualitative phytochemical analysis confirmed the presence of carbohydrates, tannins, saponins, flavonoids, quinones, glycosides, terpenoids, phenols, coumarins, steroids and phytosteroids and absence of alkaloids, phlobatannins and anthraquinones. Turpentine, the socalled resin of pine trees, is the viscous pleasantly smelling bal. Analysis of 33 primary and secondary terpenes found in. Hptlc offers better resolution and estimation of active constituents can be done with reasonable accuracy in a shorter time. In vitro antioxidant activities and hptlc fingerprint analysis of five malawian medicinal plants article pdf available january 2019 with 78 reads how we measure reads. Jrd, tata foundation of research in ayurveda and yoga sciences, deendayal research.
Hptlc fingerprint analysis of the extract was carried out using camag hptlc system equipped with linomat 5 sample applicator, uv cabinet and wincats4 software. Highperformance thinlayer chromatography wikipedia. The biologically active secondary metabolites of ginkgo biloba extract egb 761 in phytopharmaceuticals were analyzed using two simple, rapid, accurate and sensitive hplc methods. The aim of this work was to elucidate the various secondary metabolites such as alkaloids, flavonoids, glycosides, saponins, and terpenoids in the methanolic leaf and root extracts of hypochaeris radicata, a most important traditional medicinal plant species in nilgiris, the western ghats, india, using high performance thin layer chromatography hptlc. Hptlc was performed using silica gel 60 f 254 plates. The proposed methods were successfully applied in the determination of terpenes and flavonoids in four phytopharmaceutical preparations selected from the egyptian market. They are constantly being produced but are vaporized by heat and light of the day so harvest in early morning. Terpenoids terpenoids are responsible for the aroma of cannabis while the cannabinoids themselves are odorless. The preliminary phytochemical analysis in our laboratory confirmed the presence of secondary metabolites in the 50% hydro ethanolic extract. Acute toxicities and effects on flight motor activity and wing beat. Pdf isolation of terpenoids constituents from lippia. Turpentine, the socalled resin of pine trees, is the viscous pleasantly smelling balsam which flows upon cutting or carving the bark and the new wood of several pine. Dcm fraction showed the maximum number of uv active compounds and was thus detected at 254 nm, whereas other fractions and mother extract were detected at 520 nm after visualization, using anisaldehyde sulphuric acid reagents figures 5a5f.
Hptlc analysis, antioxidant and antigout activity of indian. Preliminary phytochemical evaluation and hptlc fingerprinting of leaves of azadirachta indica sunita dalal. Rahul kasar1, ashish gogia 2, kartik shah2, vetriselvan1 and chinmay anand 1health foods research lab,phytochemistry, research and development, the himalaya drug company makali. Nipanikar abstract the analysis and quality control of herbal medicines are moving a step ahead towards an integrative and comprehensive direction, in order to tackle the complex nature of herbal medicines. We provide highend instruments, tools and concepts for tlc hptlc. Hptlc analysis of berberine in stem extracts of fibraurea. Qualitative and quantitative phytochemical analysis of. All the solvents used for hptlc analysis were obtained from merck. The rate of discovery of new terpenoids has increased over the last ten years largely as a result of the increase in the sophistication of separation and analytical techniques. Confirmation of isolation of terpenoids the developed plates after hptlc were then scanned for the available possible bands using the camag automatic tlc scanner 3. Tlc and hptlc fingerprint profile journal of pharmacognosy and. Quantitative analysis were also conducted to determine the amount of alkaloids, flavonoids, phenol and. Terpenoids the name terpenoids, or terpene, derives from the fact that the first members of the class were isolated from turpentine terpentin in german.
The methanolic tuber extract showed the presence of 5 alkaloids, 6 flavonoids, 2 glycosides, 10 saponins and 7 terpenoids. Phytochemical, hptlc finger print analysis and antimicrobial. Highperformance thinlayer chromatography hptlc is an enhanced form of thinlayer chromatography tlc. Department of biotechnology, bimr college of life sciences, gwalior, m. The hptlc fingerprinting of mother extract and different fractions was developed on silica gel. Hptlc analysis, antioxidant and antigout activity of.
Hptlc densitometric analysis of orthosiphon stamineus leaf. In the present study, cardiac glycosides were separated by. Sch 511 biosynthesis of terpenoids university of nairobi. The partition and purification of plant constituents is. About 140 of these belong to a large class of aromatic organic hydrocarbons known as terpenes pronounced turpeens. The qualitative analysis of methanolic leaf and root extracts of h. The identity of the quercetin bands in sample chromatograms was confirmed by the chromatogram obtained from the sample with that obtained from the reference standard solution sachin u. The cytosol localized mevalonate pathway provides c5 units for sesquiterpene and triterpene biosynthesis. Also it is useful as a phytochemical marker and a technique to check the genetic variability in various plant species. Hptlc analysis also confirmed the presence of alkaloids, steroids, flavonoids, saponins, phenols, glycosides and terpenoids. Separation and quantification of terpenoids of boswellia. Hptlc studies were carried out as per the methods of harborne and wagner et al. Isolation of terpenoids constituents from lippia nodiflora by preparative hptlc method. A number of enhancements can be made to the basic method of thinlayer chromatography to automate the different steps, to increase the resolution achieved and to.
Steroids, anthroquinone, tannin, oils and resins were absent in the extract. In hptlc, the same analysis can be viewed using different wavelengths of light thereby providing a more complete profile of the plant than is typically observed with more specific types of analysis. The terpenoids, sometimes referred to as isoprenoids, are a large and. We provide highend instruments, tools and concepts for tlchptlc. Research article hptlc fingerprints of various secondary. Camag is the world leader in instrumental thinlayer chromatography tlc hptlc. Hptlc analysis hptlc analysis of the aqueousethanol extract of culenta mycelium was carried out. The fivecarbon building blocks of all terpenoids,sch 511 isopentenyl diphosphate ipp and dimethylallyl diphosphate dmapp, are derived from two independent pathways localized in different cellular compartments. Phytochemical analysis of ethanolic extract of dichrostachys. Preliminary phytochemical screening of the bark extracts of ficus nervosa showed the presence of chemical constituents like alkaloids, glycosides, sugars and carbohydrates, flavonoids, tannins, phenolic compounds, etc. High performance thin layer chromatography hptlc it is the most recent type of tlc plate, coated with the more fine micro particles of silica that are used in the columns for hplc. A number of enhancements can be made to the basic method of thinlayer chromatography to automate the different steps, to increase the resolution achieved and to allow more accurate quantitative measurements.
Phytochemical and hptlc studies of methanolic extract of. The screening of the terpenoids with primary phytochemical screening process revealed the presence. With advances in technology in recent years, tlc has become more sophisticated and with the development of high performance thin layer chromatography hptlc, has the potential to provide both qualitative and quantitative data. Request pdf on jun 30, 2014, venkatesh r and others published phytochemical studies on the terpenoids of medicinally important plant solanum villosum mill.
Phytochemical and hptlc studies of methanolic extract of indigofera tinctoria fabaceae f. Camag is the world leader in instrumental thinlayer chromatography tlchptlc. Linear regression analysis for each of the markers was performed by external standard method. Plant terpenoids are used extensively for their aromatic qualities. Norhayati2 1school of pharmacy, university college sedaya international, taman connaught, cheras, kuala lumpur, malaysia. Hptlc analysis for phytocompounds test solution 2l and 4 l of standard solution was loaded as 6mm band length in the 4x10 silica gel 60f 254 hptlc plate using hamilton syringe and camag linomat 5 instrument. High performance thin layer chromatography hptlc and. This showed the presence of the bioactive compound flavonoid. Hptlc analysis of bioactivity guided anticancer enriched.
Quantitative hptlc analysis was performed using videoscan software camag with a minimum peak width of 7 pixel, peak heights of 400 pixel, and a minimum area of pixel. Enhancement of the separation of boswellic acids on hptlc plates was carried out by amd chromatography. Phytochemical and hptlc studies of methanolic extract of indigofera tinctoria fabaceae. Vitex negundo medicinal plant anylisis by morden hptlc. Several terpenoids are present in cannabis plant material and would include pinene. The carbon skeletons of open chain monoterpenoids and sesquiterpenoids are, chemistry of terpenoids t research article. Preliminary phytochemical evaluation and hptlc fingerprinting. Evaluation of biochemical contents, trace elements. Hptlc is the most simple separation technique available to the analyst16. Hptlc would be suitable technique for resolving the problem because of its highly accurate data. Structural elucidation of terpenoids by spectroscopic. Phytochemical screening, hptlc fingerprint analysis of.
Hplc determination of certain flavonoids and terpene. The words terpene and terpenoid are increasingly used interchangeably, although these terms do have different meanings. Fingerprint analysis approach using highperformance thinlayer chromatography hptlc has become the most potent tool for quality control of herbal medicines because of its simplicity and. The chemical fingerprinting were carried out by high performance thin layer chromatography hptlc, antioxidant activity by abts, dpph, frap radical scavenging assays, and antiogout activity. Phytochemical screening, hptlc fingerprint analysis of leaf. In applying isoprene rule we look only for the skeletal unit of carbon. The samples 2l were spotted in the form of bands of length 6mm with a camag microlitre. The development of such fingerprint can be used in differentiation of the species from the adulterant in terms. Journal of pharmaceutical analysis critical factor that governs a successful tlc hptlc analysis of herbal medicinal products hmps. The cannabis plant consists of a wide variety of chemicals and compounds. Developed is a gcfid method with excellent resolution and peakshape for 33 primary and secondary terpenes found in cannabis. Research article hptlc finger print profile and gomphrena. Physicochemical and phytochemical analysis of bauhinia variegata modern analytical hptlc fingerprinting.
The hptlc analysis, antioxidant, and antigout activity of asparagus racemosus, withania somnifera, vitex negundo, plumbago zeylanica, butea monosperma and tephrosia purpurea extracts were investigated. Results and discussion screening of terpenoids the initial screening of the terpenoids in the plant extract with basic preliminary procedures was the first step in the process. Hptlc fingerprinting analysis of evolvulus alsinoides l. In vitroantioxidant activity and hptlc determination of nhexane. All terpenoids are derived by repetitive fusion of branched fivecarbon units based on isopentane skeleton. The plates after application of the sample were developed in twin trough chamber. High performance thin layer chromatography hptlc and high. Figure 2a and 2b show the hptlc profiles of the markers sen and tmf and os samples. Excellent linearity was observed between peak areas and concentrations r20. Terpenoids, also known as isoprenoids, are the most numerous and structurally diverse natural products found in many plants. Azadirachta indica, hptlc fingerprinting, solvent system, phytochemical analysis. Hptlc study confirms the presence of the flavonoid quercetin by comparing the total.
Muthulingam department of zoology, faculty of science, annamalai university, annamalainagar, tn india abstract the aerial parts fresh leaves of the plant indigofera tinctoria were collected powdered and the powder was initially. Hence, an attempt has been made to give a complete hptlc profile for leaf, stem, root and flower of asclepias curassavica l. The chemical fingerprinting were carried out by high performance thin layer chromatography hptlc, antioxidant activity by abts, dpph, frap. Plants are endowed with free radical scavenging molecules, such as vitamins, terpenoids, phenolic acids, lignins, stilbenes, tannins, flavonoids. The hptlc method for routine quality control of present species can be carried out using this method for extracts of plant and serve in qualitative, quantitative and was appropriate for standardization of the extract. The profiles of various individual secondary metabolites were made and developed for authentication. Tlc and hptlc fingerprint profiles of different bioactive components from the tuber of solena amplexicaulis. Phytochemical screening and hptlc fingerprint analysis of.
Analysis of all fractions of seed extract revealed the presence of carbohydrates, proteins, alkaloids, terpenoids, steroids and cardiac glycosides as primary and secondary metabolites. Isolation of terpenoids constituents from lippia nodiflora by. This solution was used as a test solution for hptlc analysis. It is a major advancement of tlc principle with short time duration and better resolution. Hptlc finger print analysis of the ficus nervosa bark extracts. Hptlc analysis of berberine in stem extracts of fibraurea darshani the present study is a first report on the phytochemical analysis of the plant fibraurea darshani which is endemic to western ghats. Structural elucidation of terpenoids by spectroscopic techniques g. The well resolved hptlc profiles also showed the occurrence of these metabolites of medicinal importance which support the traditional therapeutic uses of this species. The results from hptlc fingerprint analysis of the ethanolic leaf extract of bridelia. Hptlc densitometric analysis of orthosiphon stamineus leaf extracts and inhibitory effect on xanthine oxidase activity g. The proposed hptlc method for the simultaneous quantification of the major boswellic acids ba, aba, kba, and akba was found to be simple, precise, specific, sensitive, and accurate and can be used for routine quality control and for the quantification of these compounds in plant materials. Manasa lecturer in chemistry, gdc w, karimnagar, corresponding author. Neelamegam2 1, 2 department of botany and research centre,s.